| FAM3D

Family with sequence similarity 3, member D is a protein that is encoded by the FAM3D gene.FAM3D is a Protein Coding gene. Diseases associated with FAM3D include Deafness, Autosomal Recessive 17 and Diamond-Blackfan Anemia 4. Among its related pathways is Pancreatic cancer subtypes. Gene Ontology (GO) annotations related to this gene include cytokine activity. An important paralog of this gene is FAM3A.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	20	10	5	2.5	1.25	0.625	0.312	0
OD	2.29	1.29	0.71	0.43	0.29	0.2	0.18	0.07
Corrected OD	2.22	1.22	0.64	0.36	0.22	0.13	0.11	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human FAM3D spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	82-101	96
Cell Lysates(n=8)	96-109	105

 

| Linearity

Samples were spiked with high concentrations of Human FAM3D and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Tissue Homogenates(n=4)	Cell Lysates(n=4)
1 : 2	Range (%)	84-95	90-110
	Average (%)	90	96
1 : 4	Range (%)	89-103	105-115
	Average (%)	94	108